What are Lipid Nanoparticles (LNPs)?
Lipid nanoparticles (LNPs) are a class of nanoparticles that are used for drug delivery. As the name suggests, these couriers are nano-sized particles that are composed of lipids. They are porous spheres composed of various types of lipids that encapsulate therapeutics and vaccines. The size of LNPs can range from 10 to 1000 nm with the diameter being inversely proportional to their hydrophobicity. The surface area to volume ratio of these nanoparticles is very high and this allows them to carry many different types of components.
The first attempts to use these particles as drug delivery carriers were described in 1978, where exogenous mRNA was delivered into host cells using liposomes (1, 2). More recently, cationic lipids, ionizable lipids, among other types of lipids were employed in various LNP formulations for nucleic acid delivery.
The siRNA-LNP drug (Onpattro) was the first LNP formulation to achieve a significant regulatory milestone by gaining FDA approval for the treatment of polyneuropathy (3). This was followed by a paradigmatic shift in the use of these formulations after the significant success these platforms achieved in the development of vaccines against the novel SARS-CoV-2 virus that causes COVID19 .
Prorenata Biotech has optimised formulation techniques to formulate similar LNPs to all approved formulations with superior characteristics and consistency. Get in touch with us if you would like to discuss how you can test these formulations for your genomic medicine.
Lipid nanoparticles are an important tool in nanomedicine. They can be used to deliver mRNA, siRNA, and other oligonucleotides. LNPs can also be used to deliver small molecules, vaccines, and drugs to cells with high precision. The ability to specifically target cells with lipid nanoparticles has made them a valuable tool in the treatment of cancer and other diseases. Prorenata Biotech’s team are experienced in tailoring LNPs to various applications. We develop our formulations with the scalability in mind, so you can move your therapeutics seamlessly to the next stages of development.
Lipid nanoparticles has been implemented in vaccines against COVID19, and are also being investigated as a delivery system for other vaccines. Clinical trials are underway to test the safety and efficacy of lipid nanoparticle-based vaccines against a variety of diseases, including influenza, HIV, and malaria. Lipid nanoparticles have the potential to revolutionize the way we treat disease by providing a more targeted and effective approach to medicine.
At Prorenata Biotech, we believe that the potential of lipid nanoparticles is far beyond the development of vaccines only. We strive to develop Novel technologies to improve the targeting capabilities of these particles to take your medicines to where it should really be.
LNPs have several advantages over conventional drug delivery systems. They can be tailored for specific applications by varying the composition and molecular weight of the lipid bilayer, which determines its permeability and rate of dissolution. They can also be bound with targeting ligands to increase their specificity and improve their uptake by target organs. This makes them extremely versatile and allows them to be used in a wide range of applications.
- DIMITRIADIS, G. (1978). Translation of rabbit globin mRNA introduced by liposomes into mouse lymphocytes. Nature, 274(5674), 923-924. doi: 10.1038/274923a
- OSTRO, M., GIACOMONI, D., LAVELLE, D., PAXTON, W., & DRAY, S. (1978). Evidence for translation of rabbit globin mRNA after liposomemediated insertion into a human cell line. Nature, 274(5674), 921-923. doi: 10.1038/274921a0
- Akinc, A., Maier, M., Manoharan, M., Fitzgerald, K., Jayaraman, M., & Barros, S. et al. (2019). The Onpattro story and the clinical translation of nanomedicines containing nucleic acid-based drugs. Nature Nanotechnology, 14(12), 1084-1087. doi: 10.1038/s41565-019-0591-y
Prorenata Biotech in the Novel Space of Lipid Nanoparticles
Paving your pathway to the clinics
We are establishing a state-of-the-art GMP facility to streamline the process of moving your therapeutics to the clinics. We aim to provide you with the necessary tools to help you every step of the way. With our years of experience, we will be there to guide you and make sure your project is a success.
Custom and novel formulations
We understand that each therapeutic is different and requires a unique approach. That’s why we offer custom and novel formulations for our Lipid Nanoparticles. Whether you are developing vaccines, anticancer agents, or your application requires improved organ targeting, you’ve come to the right place. Our team of experts are on hand to provide support every step of the way.
Molecular biology to drug delivery
We provide end-to-end services and modular solutions to support your project each step of the way. We will help you along the journey of developing your therapeutics and vaccines, starting from the early stages of discovery, conceptualization and construct designing, through to testing and validation, and all the way to the development of scalable platforms ready for clinical trials. We are experts in gene regulation, molecular biology, and drug delivery platforms. Talk to us today to discuss how we can make your project a success.
Determining the right parameters for your lipid nanoparticle (LNP) formulation can be complex. Our user-friendly LNP Formulation Genii does the work for you.
This free online tool allows you to instantly calculate microfluidic mixing volumes, ratios, concentrations, and conditions based on your construct, lipid, and formulation requirements.
Get recommended parameters in seconds to optimise your LNP for delivery of mRNA, siRNA, plasmids, and more. The LNP Formulation Genii simplifies the first step in developing an effective delivery system.
Then go beyond mixing parameters. Our team of experts can further enhance your LNPs for stability, targeting, safety, and scalability. Contact us to discuss tailored formulation optimisation and production solutions for your gene therapy goals.